De Vos, and J. The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus is a unique glycolytic enzyme that belongs to the cupin superfamily. The vials were then cooled on ice for 5 min, and the residual enzyme activity was tested and compared to the controls unheated samples. The lecture panel at PGIS well experienced and very helpful and they encourage the students to reach greater heights. Not surprisingly, cPGI family sequences cannot be divided into a bacterial and an euryarchaeal cluster but rather into two or three subfamilies: Comparison of molecular and kinetic properties of cPGIs from P.

I really valued the support and encouragement received from lecturers and my colleagues. Support Center Support Center. Thus, for the respective proteobacterial cpgi genes, a lateral gene transfer from euryarchaeal species has to be assumed, since a loss of this gene in almost all bacteria appears to be unlikely. Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litorali s. Reactivation of cPGIs from P. Determination of metal content.

PGI plays a central role in sugar metabolism of eukarya, bacteria, and archaea, both in glycolysis via the Pgs pathway in eukarya and bacteria and in its modified versions found in archaea.

pgis thesis format

After the column was washed with 60 ml of buffer B and ml of buffer C 50 mM piperazine [pH 5. Confidence limits were estimated by bootstrapping replicates. Their function as cPGIs has been described either recently 22pgia52 or in the present study. The given pH values correspond to the respective temperature used. The X-ray crystal structure of phosphomannose isomerase from Candida albicans at 1.


Pgis thesis format outline

The genome of Methanosarcina mazei: Support Center Support Center. Under these conditions activity remained about constant. Thus, cPGIs are either monomeric or dimeric monocupins. Glucosephosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: The proposed origin of the cPGI family in the euryarchaeal evolution is indicated by an open circle, and the postulated lateral gene transfers are indicated by arrows.

Postgraduate Institute of Science

Among the substances tested, only sorbitolphosphate had a significant effect on the cPGIs with K i values ranging from 0. The inserted gene sequences were thesia on each strand by the method of Sanger et al. Sc in Computer Science. Crystal structure of human phosphoglucose isomerase and analysis of the initial catalytic steps. This includes all proteobacteria, especially Agrobacterium tumefaciens and S. Their presence is presumably the result of a lateral gene transfer from a Thermococcus -like species after the lines of E.

Postgraduate Institute of Agricultur

Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Glucosephosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga theesis Indeed, in a phylogram including both cPGIs and several putative members of the cupin superfamily from eukaryal, bacterial, and archaeal cPGIs form a separate cluster within the cupin superfamily not shown and thus represents its own cPGI family.

pgis thesis format

Thus far, conclusive biochemical studies analyzing the role of metals have not been performed. Archaeoglobus fulgidus and Methanosarcina mazei. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.


pgis thesis format

The latter assay was routinely pvis for most PGI activity determinations. In most cases the corresponding metal-binding site of other cupin superfamily proteins is coordinated by three histidines and a glutamate as well Phylogenetic analysis suggests lateral gene transfer for the bacterial cPGIs from euryarchaeota.

Transkingdom transfer of the phosphoglucose isomerase gene.

All metals were used in the form of chlorides. The unique features of glycolytic pathways in Archaea. In this report we pigs a comprehensive characterization of cPGIs from archaea and bacteria which represent a novel PGI family. Enzyme assays and determination of fhesis parameters. Evolution of functional diversity in the cupin superfamily. Spectra were recorded in 2-mm cuvettes and corrected for the signal of the solvent 10 mM sodium phosphate [pH 7.

The lecture panel at PGIS well experienced and very helpful and they encourage the students to reach greater heights. Crystal structure of rabbit phosphoglucose isomerase complexed with 5-phospho-d-arabinonate identifies the role of Glu in catalysis.

The measured K Me,0.